Anti-AFM Rabbit Polyclonal Antibody for WB, ELISA - P43652

Core Product Specifications and Parameters

Biological Background: Afamin Function and Localization

• Afamin (AFM), also known as alpha-albumin (ALB2, ALBA), is a member of the albumin gene family.
• It functions as a carrier for hydrophobic molecules in body fluids, essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A, and WNT10B. Related references: PMID:26902720
• Binds vitamin E and may transport it in body fluids when the lipoprotein system is insufficient. Related references: PMID:12463752, PMID:15952736
• May be involved in the transport of vitamin E across the blood-brain barrier. Related references: PMID:19046407
• The protein is secreted and is detected at high levels in plasma, as well as in extravascular fluids such as follicular and cerebrospinal fluids.
• Post-translational modifications include glycosylation and disulfide bonds, consistent with its role as a secreted carrier protein.
• It contains characteristic albumin-type structural repeats.

Experimental Guidance and Technical Tips

• For WB, consider a primary antibody dilution range of 1:500–1:2000, and validate using a positive control expressing afamin (expected band ~69 kDa).
• Since the immunogen covers amino acids 260–599, the antibody may recognize both native and denatured afamin; verify under your specific assay conditions.
• For ELISA, pair this antibody with a suitable detection antibody or use it as a capture reagent for afamin quantitation in plasma, follicular fluid, or cell culture supernatants.
• The reported cross-reactivity with rat suggests epitope conservation; validate performance in rat samples if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents—from concept to clinic. For afamin-related diagnostics or research, we can support your projects with high-quality AFM antibodies, recombinant proteins, and rare target sourcing. Whether you need routine supply or custom development, CamelBio bridges your IVD raw material needs efficiently.