Anti-AFG3L2 Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q9Y4W6

Core Product Specifications and Parameters

Biological Background: AFG3L2 Function and Localization

• AFG3L2 is a catalytic component of the mitochondrial inner membrane m-AAA protease, essential for axonal and neuron development.
  Related references: PMID:19748354, PMID:28396416, PMID:29932645

• The m-AAA protease performs quality control by degrading misfolded mitochondrial inner membrane proteins.
  Related references: PMID:26504172, PMID:30683687, PMID:34718584

• AFG3L2 processes and matures mitochondrial proteins including MRPL32, PINK1, and SP7.
  Related references: PMID:22354088, PMID:29932645, PMID:30252181

• It regulates mitochondrial calcium homeostasis by degrading SMDT1/EMRE, thereby controlling MCU complex assembly.
  Related references: PMID:27642048, PMID:28396416

• Under hyperpolarization, AFG3L2 degrades GHITM, leading to respiratory complex I remodeling.
  Related references: PMID:35912435

• The protease is a key regulator of mitochondrial glutathione and carnitine metabolism via SLC25A39 and SLC25A45 cleavage.
  Related references: PMID:37917749, PMID:38157846, PMID:41075794

• AFG3L2 is involved in OMA1-dependent OPA1 processing, crucial for mitochondrial dynamics.
  Related references: PMID:17615298, PMID:29545505, PMID:30252181

• Located in the mitochondrial inner membrane, AFG3L2 is ubiquitously expressed with high levels in cerebellar Purkinje cells.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 538-797), enabling detection of both full-length and potentially processed AFG3L2 forms.
• For Western blot, consider enriching mitochondrial fractions from cell or tissue lysates to improve sensitivity.
• When performing IHC-P, optimize antigen retrieval conditions; citrate or EDTA buffer may be suitable for formalin-fixed paraffin-embedded tissues.
• Validate the antibody in species (human, mouse, rat) and appropriate positive controls, such as neuronal or muscle cell lines known to express AFG3L2.
• The predicted molecular weight is ~89 kDa, but processed or cleaved bands may appear depending on sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to high-quality IVD raw materials. This anti-AFG3L2 antibody supports mitochondrial proteostasis and neurodegenerative disease research, and CamelBio can supply validated antibody pairs, bulk ancillary reagents, and optimized polyclonal and monoclonal antibodies tailored to your assay development needs. For rare target sourcing or custom production, our one-stop platform bridges the gap from concept to clinic.