Anti-AFG3L2 Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q9Y4W6

Core Product Specifications and Parameters

Biological Background: AFG3L2 Function and Localization

• AFG3L2, also known as AFG3-like protein 2 or paraplegin-like protein, is a mitochondrial inner membrane m-AAA protease component encoded by the AFG3L2 gene.
• It possesses both ATPase and protease activities, unfolding substrates and threading them into the proteolytic cavity for hydrolysis. Related references: PMID:19748354 PMID:31327635
• The m-AAA protease mediates degradation of misfolded or mistranslated inner membrane proteins, contributing to mitochondrial quality control. Related references: PMID:26504172 PMID:30683687 PMID:34718584
• AFG3L2 promotes the processing and maturation of mitochondrial proteins such as MRPL32, PINK1, and SP7, and is required for SPG7 maturation. Related references: PMID:22354088 PMID:29932645 PMID:30252181
• It regulates calcium homeostasis by degrading SMDT1/EMRE before its assembly with the uniporter complex. Related references: PMID:27642048 PMID:28396416
• AFG3L2 is involved in mitochondrial glutathione homeostasis through cleavage of SLC25A39, a process inhibited by iron-sulfur binding. Related references: PMID:37917749 PMID:38157846
• It also regulates carnitine biosynthesis via cleavage of SLC25A45. Related references: PMID:41075794
• AFG3L2 is ubiquitously expressed with high levels in cerebellar Purkinje cells, localizes to the mitochondrial inner membrane, and disease-associated mutations are linked to spinocerebellar ataxia type 28 and spastic ataxia 5.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 538-797) corresponds to the C-terminal region of human AFG3L2, which includes part of the protease domain; this design may favor detection of full-length and cleaved forms.
• For Western blot, a band at ~89 kDa is expected; consider using mitochondrial extracts or cell lines expressing AFG3L2, and validate in the relevant human, mouse, or rat sample system.
• For immunohistochemistry (IHC-P), antigen retrieval conditions may need optimization; include appropriate tissue controls.
• In immunoprecipitation, confirm the interaction with known binding partners and use appropriate lysis buffers to preserve mitochondrial integrity.
• For ELISA, calibrate coating and detection conditions with recombinant AFG3L2 protein and validate cross-reactivity in target species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD manufacturers, laboratories, and research institutes, offering validated antibodies, antigen pairs, and ancillary reagents to accelerate mitochondrial research and neurodegeneration studies. This anti-AFG3L2 antibody exemplifies our high-quality polyclonal portfolio, and we can support bulk orders, customization, and complementary raw materials such as recombinant proteins and assay controls. Contact our team to streamline your diagnostic development pipeline from concept to clinic.