Anti-AFF4 Rabbit Polyclonal Antibody for WB, ELISA - Q9UHB7

Core Product Specifications and Parameters

Biological Background: AFF4 Function and Localization

• AFF4 is a key component of the super elongation complex (SEC), which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing at multiple sites along the DNA.
• Within the SEC, AFF4 functions as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2, or ELL3) and the P-TEFb complex.
• In HIV-1 infection, the viral Tat protein recruits the SEC complex to stimulate viral gene expression.
• AFF4 localizes to the nucleus and associates with chromosomes.
• The protein is ubiquitously expressed, with highest levels in heart, placenta, skeletal muscle, and pancreas; lower expression is observed in brain.
• Keywords from UniProt indicate that AFF4 undergoes acetylation, phosphorylation, and ubiquitination; it is associated with chromosomal rearrangement, dwarfism, obesity, and acts as a proto-oncogene.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–220) of human AFF4; consider that epitope location may affect detection of isoforms or cleavage products.
• For Western blot (WB), the predicted molecular weight is approximately 127 kDa; validate band size in your specific sample system.
• This antibody is reported to react with human and mouse samples; for other species, verify target sequence homology before use.
• In ELISA, the antibody may serve as a capture or detection reagent; optimization in sandwich formats is recommended.
• Given the ubiquitous expression of AFF4, choose relevant positive and negative controls for your experimental design.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio efficiently sources validated antibodies like anti-AFF4 for transcription research and oncology applications, supporting diagnostic manufacturers, labs, and institutes with one-stop procurement—from antibody pairs to bulk ancillary reagents—to streamline your assay development from concept to clinic.