Anti-AES Rabbit Polyclonal Antibody for WB, ELISA - Q08117

Core Product Specifications and Parameters

Biological Background: AES/TLE5 Function and Localization

• TLE family member 5 (AES/TLE5) is a transcriptional corepressor that acts as a dominant repressor of other TLE family members.
• Inhibits NF-kappa-B-regulated gene expression and may be required for initiation and maintenance of the differentiated state.
• Essential for the transcriptional repressor activity of SIX3 during retina and lens development.
• Localizes predominantly to the nucleus, consistent with its role as a transcriptional regulator.
• Expressed in a tissue-specific manner: found in adult muscle, heart, and placenta; in fetal tissues, abundant in heart, lung, kidney, brain, and liver.
• Belongs to the Wnt signaling pathway and is classified under keywords: Alternative splicing, Nucleus, Phosphoprotein, Repressor, Transcription regulation, and Ubl conjugation.
• Protein weight is approximately 22 kDa (UniProt: 21970 Da), and alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• For Western blot, a band around 22 kDa is expected; validate the antibody with appropriate positive control lysates from relevant tissues or cell lines.
• For ELISA, the antibody can be used as a capture or detection reagent; optimize coating concentrations and blocking conditions experimentally.
• As a polyclonal antibody, it may offer high sensitivity; confirm specificity using knockdown or knockout controls to rule out cross-reactivity.
• Since the antibody is raised in rabbit, ensure use of anti-rabbit secondary conjugates for detection.
• For best results, titrate the antibody in your specific application and use the manufacturer's recommended dilutions as a starting point.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to high-quality IVD raw materials. This anti-AES antibody supports studies in transcriptional regulation and developmental biology. Beyond individual antibodies, CamelBio offers matched antibody pairs, bulk reagents, and customized sourcing services to accelerate your assay development from concept to clinic.