Anti-AEBP2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q6ZN18

Core Product Specifications and Parameters

Biological Background: AEBP2 Function and Localization

• AEBP2, also designated Zinc finger protein AEBP2 or Adipocyte enhancer-binding protein 2, is a chromatin-associated transcriptional regulator.
• It serves as an accessory subunit of the Polycomb repressive complex 2 (PRC2) and is essential for histone H3 lysine 27 trimethylation (H3K27me3), a hallmark of gene silencing. Related references: PMID:15225548, PMID:29499137, PMID:31959557
• AEBP2 facilitates proper nucleosome positioning of PRC2 on chromatin, enhancing its repressive function. Related references: PMID:29499137
• The protein localizes to the nucleus, consistent with its role in chromatin regulation.
• It contains multiple C2H2-type zinc-finger domains and is predicted to bind DNA, contributing to target gene specificity.
• Post-translational modifications include acetylation and phosphorylation, which may modulate its activity or protein interactions.
• Alternative splicing generates multiple isoforms, though their functional divergence remains unclear.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 234-503 of human AEBP2; verify reactivity in mouse and rat systems, as cross-reactivity is reported but may vary with assay conditions.
• For Western blotting, a band near 54 kDa is anticipated; include molecular weight markers and loading controls for accurate assessment.
• For immunohistochemistry (paraffin-embedded sections), optimize antigen retrieval and antibody dilution to achieve clear staining.
• In ELISA, titrate the antibody and adjust coating and detection conditions for optimal performance.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio offers an integrated IVD raw material supply chain, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For researchers studying epigenetics and Polycomb complexes, we facilitate sourcing of rare targets such as AEBP2 antibodies. Our one-stop services support your development from concept to clinic.