Anti-AdSS 2 Rabbit Monoclonal Antibody for WB and ELISA - P30520

Core Product Specifications and Parameters

Biological Background: AdSS 2 Function and Localization

• AdSS 2, also known as adenylosuccinate synthetase isozyme 2, acidic isozyme, or liver isozyme (L-type adenylosuccinate synthetase), is encoded by the ADSS2 gene.
• It catalyzes the first committed step in the biosynthesis of AMP from IMP, playing a critical role in both the de novo pathway and the salvage pathway of purine nucleotide biosynthesis.
• The enzyme functions as a ligase, requiring GTP binding and magnesium as a metal cofactor for its activity.
• Subcellularly, AdSS 2 localizes to both the cytoplasm and the mitochondrion.
• It is a member of the adenylosuccinate synthetase family, with the acidic isozyme (ADSS2) predominantly expressed in the liver.
• The protein has a molecular weight of approximately 50,097 Da (confirmed by UniProt), consistent with the observed 50 kDa band in experimental settings.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal 1‑180 amino acid region of human AdSS 2; this region is likely to recognize the full‑length protein in denaturing Western blot conditions.
• For WB, a primary band at ~50 kDa should be expected; consider validating the antibody in relevant human, mouse, or rat tissue/cell lysates known to express ADSS2.
• For ELISA, the antibody may be used as a capture or detection reagent; optimal coating and detection conditions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD developers and researchers with a comprehensive portfolio of raw materials for purine metabolism and nucleotide biosynthesis studies. Beyond this anti‑AdSS 2 monoclonal antibody, we offer validated antibody pairs, bulk ancillary reagents, and sourcing of hard‑to‑find targets to streamline your assay development pipeline. Contact us to explore how our one‑stop raw‑material solutions can accelerate your project from concept to clinic.