Anti-ADSL Polyclonal Antibody for WB, IF/ICC, ELISA - P30566

Core Product Specifications and Parameters

Biological Background: ADSL Function and Localization

• Adenylosuccinate lyase (ADSL), also known as adenylosuccinase (ASase), is a key enzyme in the de novo purine biosynthetic pathway.
• It catalyzes two non-sequential reactions: the conversion of SAICAR to AICAR plus fumarate in the IMP synthesis pathway, and the conversion of succinyladenosine monophosphate (SAMP) to AMP and fumarate.
• ADSL is encoded by the ADSL gene (GeneID: 158) and is ubiquitously expressed across human tissues; both isoforms are produced, with isoform 2 being approximately 10-fold less abundant than isoform 1.
• The protein undergoes post-translational modifications including acetylation and isopeptide bond formation, and it has been identified by proteomics and direct protein sequencing.
• Mutations in ADSL are associated with adenylosuccinase deficiency, a disorder characterized by epilepsy and other neurological symptoms, highlighting its importance in purine metabolism.
• The protein belongs to the lyase family and has a calculated molecular weight of approximately 55 kDa (UniProt: 54889 Da), consistent with the immunogen design.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using standard blocking and detection protocols; the predicted band size of ~55 kDa may require appropriate gel percentage and transfer conditions.
• Immunofluorescence/immunocytochemistry (IF/ICC) may benefit from optimization of fixation and permeabilization steps, as well as antibody titration to reduce background.
• ELISA applications can be explored for quantifying ADSL in cell or tissue lysates; pairing with a capture antibody from a different species or epitope may improve assay performance.
• The antibody is polyclonal and cross-reacts with human, mouse, and rat samples; validate in the relevant species and sample type to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research teams with validated antibody pairs, optimized polyclonal antibodies like this anti-ADSL product, and bulk ancillary reagents. We facilitate procurement across the full development pipeline, from early research on purine metabolism to clinical assay scale-up, and can source rare-target raw materials upon request. Let CamelBio streamline your supply chain so you can focus on innovation.