Anti-ADRM1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q16186

Core Product Specifications and Parameters

Biological Background: ADRM1 Function and Localization

• ADRM1 is an integral component of the 26S proteasome, a large protein complex responsible for ATP-dependent degradation of ubiquitinated proteins, essential for maintaining cellular protein homeostasis. Related references: PMID:16815440 PMID:16906146 PMID:16990800
• It functions as a proteasomal ubiquitin receptor, directly recognizing and binding ubiquitin moieties on substrate proteins targeted for degradation. Related references: PMID:18497817
• ADRM1 engages and activates the deubiquitinases UCHL5 (UCH37) and PSMD14 (Rpn11), facilitating efficient substrate deubiquitination and processing. Related references: PMID:16906146 PMID:16990800 PMID:17139257
• The protein is also known as Rpn13 (proteasome regulatory particle non-ATPase 13), adhesion-regulating molecule 1 (ARM-1), and Gp110.
• It localizes to both the cytoplasm and the nucleus, consistent with its role in degrading nuclear and cytoplasmic proteins.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation, which may regulate its function.
• Through its role in the ubiquitin-proteasome system, ADRM1 participates in diverse processes such as cell cycle progression, apoptosis, and DNA damage repair. Related references: PMID:16815440 PMID:16906146 PMID:16990800

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody corresponds to amino acids 108–407 of human ADRM1, covering a large portion of the full-length protein. This design typically yields antibodies recognizing multiple epitopes.
• For Western blotting (WB), the calculated molecular weight is 42 kDa; optimize loading controls and validate band specificity with knockdown or knockout samples if possible.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider appropriate antigen retrieval methods and primary antibody dilutions (e.g., 1:50–1:200 as a starting range) based on your sample preparation.
• Since the antibody cross-reacts with human, mouse, and rat, it is suitable for studies across these species; however, always verify specificity in your experimental context.
• For ELISA, the antibody can be used as a detection reagent after pairing with an appropriate capture antibody or antigen coating; validate with known positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing high-quality IVD raw materials, including validated antibodies like this ADRM1/Rpn13 polyclonal antibody, to support proteasome and ubiquitin research. We offer one-stop access to antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing, helping diagnostic manufacturers and researchers streamline their workflows from concept to clinic. Ensure reliable supply and consistent quality for your proteasome-related assay development.