Anti-ADRBK2 (GRK3, BARK2) Polyclonal Antibody for WB, IHC-P, ELISA - P35626

Core Product Specifications and Parameters

Biological Background: G Protein-Coupled Receptor Kinase 3 (ADRBK2/GRK3) Function and Localization

• ADRBK2/GRK3, also known as Beta-adrenergic receptor kinase 2 (Beta-ARK-2), is a serine/threonine-protein kinase that specifically phosphorylates the activated forms of G protein-coupled receptors (GPCRs).
• Phosphorylation of agonist-occupied beta-adrenergic and closely related receptors initiates beta-arrestin-mediated receptor internalization and desensitization, attenuating downstream signaling.
• Additionally, GRK3 phosphorylates ligand-bound anaphylatoxin receptors C3AR1 and C5AR1, leading to receptor desensitization. Related references: PMID:21799898
• GRK3 positively regulates the ciliary smoothened (SMO) signaling pathway by mediating phosphorylation of SMO, facilitating SMO trafficking into the cilium and stimulating SMO activity (By similarity).
• Subcellularly, GRK3 is localized at both postsynaptic and presynaptic compartments, highlighting its role in modulating neurotransmitter receptor signaling at synapses.
• Key functional keywords include ATP-binding, nucleotide-binding, kinase, transferase, and synapse, underscoring its enzymatic activity and synaptic involvement. The protein is also subject to ubiquitin-like conjugation (Ubl conjugation).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–300 of human ADRBK2, targeting the N-terminal region, which may recognize multiple isoforms or phosphorylation states.
• For Western blot (WB), expect a predominant band near 80 kDa in appropriate lysates; verify specificity using knockout or knockdown controls.
• In immunohistochemistry paraffin (IHC-P) applications, antigen retrieval and optimized antibody dilution are suggested; tissue-specific expression should be confirmed using known positive controls.
• The polyclonal nature of this antibody may offer enhanced sensitivity due to recognition of multiple epitopes; consider cross-adsorption if higher specificity is required for closely related GRK family members.
• For ELISA, the antibody can be explored as a capture or detection reagent; pairing with a matched detection antibody may improve assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material supply for diagnostic manufacturers and research laboratories, offering a comprehensive portfolio that spans from early R&D to commercial production. This anti-ADRBK2 polyclonal antibody supports GPCR signaling and desensitization studies, and we can further provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing for rare-target raw materials. Contact us to bridge your project needs with ready-to-integrate solutions.