Anti-ADRB2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P07550

Core Product Specifications and Parameters

Biological Background: ADRB2 Function and Localization

• ADRB2 is a beta-2 adrenergic receptor, a G protein-coupled receptor (GPCR) that binds catecholamines epinephrine and norepinephrine, with ~30-fold higher affinity for epinephrine. Related references: PMID:2831218 PMID:33093660 PMID:7915137
• It couples to both G(s) and G(i) proteins, activating bifurcated signaling pathways: G(s) stimulates adenylyl cyclase/PKA, while G(i) activates PI3K/Akt promoting cardioprotective effects under hypoxia/oxidative stress. Related references: PMID:2831218 PMID:7915137
• In cardiomyocytes, ADRB2 regulates contraction rate; epinephrine induces rapid contraction via G(s)/PKA, while norepinephrine-mediated activation leads to slower contraction.
• The receptor also signals independently of PKA to regulate cellular pH by modulating the Na(+)/H(+) exchanger SLC9A3. Related references: PMID:9560162
• ADRB2 localizes to the cell membrane and the Golgi apparatus.
• It undergoes various post-translational modifications, including glycosylation, palmitoylation, phosphorylation, and ubiquitination.
• The protein is a member of the GPCR family with a 7-transmembrane helix structure; its gene is ADRB2 (also known as ADRB2R, B2AR).

Experimental Guidance and Technical Tips

• For Western blot, consider the predicted molecular weight of ~46 kDa; however, glycosylation and other modifications may result in a slightly higher apparent molecular weight.
• In immunofluorescence/ICC, optimize permeabilization to access the intracellular epitope region (aa 300-400) as ADRB2 is a transmembrane protein.
• For ELISA, the antibody may be suitable for both direct and sandwich formats; validate with recombinant protein or peptide.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials and related services, bridging your projects from concept to clinic. Our anti-ADRB2 polyclonal antibody is a reliable tool for cardiovascular and metabolic research. We offer validated antibody pairs, bulk reagents, and custom sourcing for rare targets to meet your diagnostic and research needs.