Anti-ADRA2C Rabbit Polyclonal Antibody for WB, ELISA - P18825

Core Product Specifications and Parameters

Biological Background: ADRA2C Function and Localization

• Encoded by the ADRA2C gene, the protein is also known as Alpha-2C adrenergic receptor, Alpha-2 adrenergic receptor subtype C4, and Alpha-2CAR. It belongs to the G protein-coupled receptor (GPCR) family.
• ADRA2C is a receptor for catecholamines that activates G(i/o) protein signaling, resulting in inhibition of adenylyl cyclase, stimulation of ERK1/2, and suppression of voltage-gated calcium channels. Related references: PMID:2842764
• It regulates key physiological processes including blood pressure, lipolysis, and insulin release. Related references: PMID:2842764
• In the nervous system, ADRA2C mediates presynaptic feedback inhibition of neurotransmitter release at low stimulation frequencies, while ADRA2A functions at high frequencies (By similarity).
• The receptor localizes to the cell membrane.
• Structural features include disulfide bonds, glycosylation sites, and seven transmembrane helices.
• The rank order of potency for physiological agonists is epinephrine > norepinephrine > dopamine. Related references: PMID:2842764

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 240–380 in the intracellular domain; consider its potential impact on detecting membrane-bound versus intracellular receptor pools.
• For Western blotting, use reducing conditions and test a range of protein loads. Include a positive control such as human or mouse tissue lysates known to express ADRA2C (e.g., brain, kidney).
• For ELISA, optimize coating concentrations using the recombinant protein or cell lysates, and confirm specificity via pre-incubation with the immunogen.
• Because polyclonal antibodies may show lot-to-lot variation, validate each new lot for your specific assay and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this ADRA2C polyclonal antibody as part of a comprehensive portfolio of IVD raw materials for GPCR and cardiovascular research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline diagnostic development. Our one-stop sourcing capabilities support you from target discovery to clinical application.