Anti-ADRA2A Rabbit Polyclonal Antibody for WB - P08913

Core Product Specifications and Parameters

Biological Background: ADRA2A Function and Localization

• ADRA2A encodes the alpha-2A adrenergic receptor, a G protein-coupled receptor that primarily couples to Gi/o proteins. Its activation inhibits adenylyl cyclase, stimulates ERK1/2, and suppresses voltage‑gated calcium channels (PubMed:2170371, PubMed:23105096, PubMed:2568356). Related references: PMID:2170371, PMID:23105096, PMID:2568356
• The receptor plays critical roles in blood pressure regulation, lipolysis, and insulin secretion (PubMed:2568356, PubMed:27376152). Related references: PMID:2568356, PMID:27376152
• It mediates presynaptic feedback inhibition of neurotransmitter release in sympathetic and central nervous systems.
• ADRA2A is localized to the cell membrane and undergoes post‑translational modifications, including glycosylation, palmitoylation, phosphorylation, and methylation, which modulate its function.
• The protein contains a conserved disulfide bond and belongs to the rhodopsin‑like GPCR family.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein corresponding to amino acids 240–380 of human ADRA2A, a region within the intracellular loops and C‑terminus; this design targets the total protein for reliable detection.
• For Western blotting, expect a major band at approximately 51 kDa; optimize antibody dilution and sample loading conditions for your specific tissue or cell line.
• This antibody is listed for ELISA; validate assay specificity using appropriate positive (e.g., overexpressed lysate) and negative controls.
• Confirmed cross‑reactivity with mouse and rat supports its use in rodent models; always verify reactivity in your own system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials. For adrenergic signaling targets such as ADRA2A, we provide validated polyclonal antibodies and can facilitate sourcing of optimized monoclonal pairs, bulk ancillary reagents, and rare‑target raw materials. Simplify your assay development—from concept to clinic—with CamelBio’s integrated procurement support.