Anti-ADRA1A Rabbit Monoclonal Antibody for WB, ELISA - P35348

Core Product Specifications and Parameters

Biological Background: ADRA1A Function and Localization

• ADRA1A (Alpha-1A adrenergic receptor) is a G protein-coupled receptor that mediates catecholamine signaling through G(q) family G proteins, activating the phosphatidylinositol-calcium second messenger pathway and protein kinase C (PubMed:37563160).
  Related references: PMID:37563160
• It binds the catecholamine ligands norepinephrine and epinephrine, and can also couple to G(14) protein, indicating diverse signaling capabilities (PubMed:18802028, PubMed:37563160, PubMed:7815325).
  Related references: PMID:18802028, PMID:37563160, PMID:7815325
• Nuclear-localized ADRA1A forms hetero-oligomers with ADRA1B to regulate phenylephrine-stimulated ERK signaling in cardiac myocytes, and at the plasma membrane, it interacts with CAVIN4/MURC to regulate ERK activation and cardiac hypertrophy (PubMed:18802028, PubMed:22120526, PubMed:24567387).
  Related references: PMID:18802028, PMID:22120526, PMID:24567387
• ADRA1A plays a critical vasopressor role in resistance arteries and is involved in maintaining normal arterial blood pressure.
• The receptor localizes to multiple subcellular compartments, including the nucleus membrane, cell membrane, cytoplasm, and caveolae, reflecting its complex signaling modalities.
• It is expressed in heart, brain, liver, and prostate, with isoform 4 being the most abundant in the prostate, liver, and heart.
• Post-translational modifications include disulfide bond formation, glycosylation, palmitoylation, and phosphorylation, which modulate receptor activity and trafficking.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 150-250 of human ADRA1A (P35348). This region lies within the third intracellular loop, which is important for G protein coupling and may affect antibody recognition of receptor conformations. Consider using denaturing conditions (e.g., WB with SDS-PAGE) to ensure access to linear epitopes.
• For Western blotting, the predicted molecular weight is ~51 kDa for the canonical isoform; however, alternative splicing and post-translational modifications (glycosylation, phosphorylation) may result in bands at higher or multiple molecular weights. Validate band specificity using appropriate controls (e.g., blocking peptide, knockout lysates).
• In ELISA, the antibody may detect denatured or native protein depending on coating conditions. Optimize coating buffer and antigen presentation to match the assay format.
• Cross-reactivity is reported for human and mouse; confirm reactivity in your experimental species if other than these. Endogenous ADRA1A expression levels may vary by tissue; consider using over-expression systems for initial detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted IVD raw-material sourcing partner for diagnostic manufacturers and research labs, offering validated antibodies, bulk ancillary reagents, and rare target materials. This anti-ADRA1A antibody supports GPCR-focused assay development, and our supply network can provide matched antigen, antibody pairs, and technical consulting to streamline your project from concept to clinic. Explore partnering with us for reliable access to critical reagents.