Anti-ADORA2B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P29275

Core Product Specifications and Parameters

Biological Background: ADORA2B Function and Localization

• ADORA2B is a G protein-coupled adenosine receptor that binds adenosine to activate adenylyl cyclase, increasing intracellular cAMP levels.
• It is a multi-pass membrane protein localized to the cell membrane.
• The receptor contains seven transmembrane helices and features conserved disulfide bonds and palmitoylation sites, characteristic of class A GPCRs.
• Post-translational modifications include N-linked glycosylation and S-palmitoylation, which may regulate receptor expression and signal transduction.
• ADORA2B has a calculated molecular weight of approximately 36.3 kDa (UniProt P29275), consistent with an observed band around 36 kDa in western blotting.
• The human ADORA2B gene (GeneID: 136) encodes the primary sequence (NP_000667.1) and is known by aliases ADORA2 and ADORA2B.

Experimental Guidance and Technical Tips

• For western blotting, expect a band at ~36 kDa; consider using membrane protein extraction protocols and avoid excessive boiling to prevent aggregation.
• In immunofluorescence/ICC, permeabilize cells to allow antibody access to the intracellular epitope (aa 100-200); include peptide blocking controls to confirm specificity.
• For ELISA applications, validate the antibody as a capture or detection reagent using recombinant ADORA2B protein or immunizing peptide.
• Although cross-reactivity with mouse and rat is indicated, verify by sequence alignment of the immunogen region for each species before quantitative assays.
• The polyclonal nature may give robust detection across denatured and native conformations, making it suitable for multiple applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, technical services, and consulting, guiding projects from concept to clinic. For adenosine receptor research and GPCR-targeted assay development, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk auxiliary reagents, and custom sourcing of rare target materials. Partner with CamelBio to streamline your raw material procurement and accelerate diagnostic innovation.