Anti-ADNP Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H2P0

Core Product Specifications and Parameters

Biological Background: ADNP Function and Localization

• Activity-dependent neuroprotector homeobox protein (ADNP), also known as ADNP1, is a member of the homeobox family of transcription factors and contains nine zinc-finger domains, suggesting a role in DNA binding and transcriptional regulation.
• ADNP may mediate some neuroprotective effects of vasoactive intestinal peptide (VIP) and positively modulate WNT-β-catenin signaling by regulating CTNNB1 phosphorylation and stability.
• It is proposed to be involved in neural induction and neuronal differentiation, and may also play a role in erythroid differentiation based on similarity to related proteins.
• Subcellular localization is predominantly nuclear and associated with chromosomes, consistent with its putative function in chromatin modulation and gene expression.
• Widely expressed in normal tissues, with strong expression in heart, skeletal muscle, kidney, and placenta; in the brain, higher expression is observed in the cerebellum and cortex.
• Notably, ADNP expression is strongly increased in colon and breast cancer tissues, while absent in normal colon, suggesting a potential role in cancer progression.
• Post-translational modifications include phosphorylation, acetylation, and methylation, which may regulate its activity and stability; it is also a target for ubiquitination and ISGylation.
• Mutations in ADNP are associated with autism spectrum disorder and intellectual disability, highlighting its critical role in neurodevelopment.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 843–1102 of human ADNP, a region likely containing functional domains; consider epitope accessibility in native versus denaturing conditions when designing experiments.
• For Western blotting, denaturing and reducing conditions are recommended; validate the observed band near 124 kDa in a suitable positive control lysate (e.g., HeLa, brain, or kidney extracts).
• In immunofluorescence/ICC, anticipate nuclear staining; fixation and permeabilization conditions should be optimized to preserve antigenicity of the target region.
• For ELISA applications, titrate the antibody against the recombinant immunogen or endogenous protein in the relevant sample system to determine optimal working concentrations.
• Cross-reactivity with mouse is reported, but sequence alignment of the immunogen region between human and mouse is advised to predict reactivity with other species.

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CamelBio provides diagnostic manufacturers and research laboratories with integrated access to IVD raw materials, connecting you with validated antibody pairs, optimized polyclonal antibodies like this anti-ADNP product, and auxiliary reagents for transcriptional and neurobiology research. Whether you need bulk quantities, rare target sourcing, or technical consultation from concept to clinic, our supply network supports your development of reproducible assays.