Anti-ADM Rabbit Polyclonal Antibody for WB and ELISA - P35318

Core Product Specifications and Parameters

Biological Background: ADM Function and Localization

• ADM, also known as AM or PAMP, is a multifunctional peptide hormone encoded by the ADM gene. It is produced as a precursor protein, pro-adrenomedullin, which is cleaved to generate several active peptides including adrenomedullin and PAMP.
• ADM exerts potent hypotensive and vasodilatory effects, primarily through interactions with the CALCRL-RAMP2 and CALCRL-RAMP3 receptor complexes. Related references: PMID:8387282, PMID:9620797, PMID:32296767
• In addition to vascular actions, ADM and PAMP regulate fluid and electrolyte balance, exhibiting diuretic and natriuretic properties in the kidney and inhibiting aldosterone secretion from the adrenal glands. Related references: PMID:8387282, PMID:9620797
• Both peptides suppress basal ACTH secretion in the pituitary gland and act in the central nervous system to promote plasma volume loss, complementing their peripheral hypotensive actions. Related references: PMID:8387282, PMID:9620797
• ADM also inhibits catecholamine release from sympathetic nerve endings and adrenal chromaffin cells, and serves as a ligand for MRGPRX2 receptors on mast cells, suggesting roles in neuroendocrine and immune modulation. Related references: PMID:10588445, PMID:9578982, PMID:15823563, PMID:34789875
• ADM is primarily secreted and shows highest expression in pheochromocytoma, adrenal medulla, lung, ventricle, and kidney tissues. Subcellularly, it is secreted extracellularly.
• Post-translational modifications include amidation, cleavage on paired basic residues, and formation of a disulfide bond, which are critical for its biological activity.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic peptide spanning amino acids 50-150 of human ADM. For Western blot, consider using denaturing and reducing conditions; the predicted molecular weight is approximately 20 kDa.
• In ELISA, the antibody may be used as a capture or detection reagent, but optimal dilutions and conditions should be determined empirically in the relevant assay system.
• Due to the secreted nature of ADM, appropriate sample types (e.g., serum, plasma, tissue lysates) and controls should be included. Cross-reactivity with mouse ADM has been observed, making it suitable for rodent models; however, validation in the specific experimental context is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw materials, offering validated antibodies, proteins, and ancillary reagents. This ADM antibody supports research in cardiovascular physiology, hypertension, and fluid homeostasis, complementing our broad range of hormone and peptide detection tools. From bulk antibodies to custom sourcing, we streamline your supply chain from concept to clinic.