Anti-ADIPOR1 Rabbit Polyclonal Antibody for WB, ELISA - Q96A54

Core Product Specifications and Parameters

Biological Background: ADIPOR1 Function and Localization

• ADIPOR1 (also known as PAQR1, TESBP1A) is a member of the progestin and adipoQ receptor (PAQR) family and functions as a high-affinity receptor for globular adiponectin.
• Upon adiponectin binding, it activates AMPK signaling, promoting increased fatty acid oxidation, glucose uptake, and decreased gluconeogenesis, thereby regulating glucose and lipid metabolism (PubMed:12802337, PubMed:25855295).
  Related references: PMID:12802337, PMID:25855295
• ADIPOR1 localizes to the cell membrane and contains seven transmembrane domains, characteristic of the PAQR family.
• It is widely expressed, with highest levels in heart and skeletal muscle, intermediate in brain, spleen, kidney, liver, placenta, lung, and peripheral blood leukocytes, and low in colon, thymus, and small intestine (PubMed:12802337, PubMed:16044242).
  Related references: PMID:12802337, PMID:16044242
• The protein binds zinc ions and has a known three-dimensional structure, as indicated by UniProt keywords "Zinc" and "3D-structure".
• ADIPOR1 is critical for maintaining normal body weight and whole-body insulin sensitivity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1–136 amino acids of human ADIPOR1, so the antibody may preferentially recognize the full-length protein or N-terminal fragments.
• For WB, a band at approximately 43 kDa is expected in reducing conditions. Validate using lysates from tissues with high ADIPOR1 expression (e.g., heart, skeletal muscle).
• In ELISA, the antibody can be employed for detecting recombinant ADIPOR1 or endogenous protein in cell/tissue extracts; consider appropriate coating and detection conditions.
• Cross-reactivity with mouse and rat has been reported, making it suitable for studies in these species, but always confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a reliable one-stop supplier of IVD raw materials, offering validated antibodies, recombinant proteins, and ancillary reagents for metabolic disease research. Our ADIPOR1 antibody supports studies on adiponectin signaling and insulin sensitivity, facilitating diagnostic assay development. Contact us for custom sourcing solutions tailored to your project needs.