Anti-Adiponectin Rabbit Monoclonal Antibody for WB and ELISA - Q15848

Core Product Specifications and Parameters

Biological Background: Adiponectin Function and Localization

• Adiponectin, also known as ACRP30, APM-1, and GBP28, is a member of the C1q and collagen domain-containing protein family (Q15848).
• It is an important adipokine secreted exclusively by adipocytes and circulating in plasma as low, medium, and high molecular weight complexes.
• Adiponectin plays a key role in regulating glucose and lipid metabolism, enhancing insulin sensitivity via AMPK phosphorylation in liver and skeletal muscle.
• It antagonizes TNF-alpha signaling by reducing its expression and counteracting its pro-inflammatory effects in macrophages and endothelial cells.
• Through a cAMP-dependent pathway, it inhibits endothelial NF-kappa-B signaling, contributing to its anti-atherogenic activities.
• Adiponectin may also modulate cell growth, angiogenesis, and tissue remodeling by binding and sequestering various growth factors.
• Keywords associated with this protein include collagen domain, hormone, secreted, glycoprotein, and involvement in diabetes and obesity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature secreted form (aa 19–244), so the antibody is expected to recognize both endogenous and recombinant adiponectin.
• For Western blotting, consider using human or mouse adipocyte lysates as a positive control; the monomeric form migrates near 26 kDa, but higher-order multimers may be evident.
• ELISA-based assays may require optimization of coating conditions and standard curves due to the multimeric nature of the protein.
• Cross-reactivity with mouse allows for dual-species studies; validate performance in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For metabolic and obesity-related biomarker research, our adiponectin antibody portfolio includes validated clones and antibody pairs suitable for immunoassay development. We also supply bulk ancillary reagents and can source rare targets to accelerate your diagnostic workflows.