Anti-Adiponectin Polyclonal Antibody for WB, IHC-P, ELISA - Q15848

Core Product Specifications and Parameters

Biological Background: Adiponectin Function and Localization

• Adiponectin is a secreted adipokine (also known as ACRP30, apM-1, GBP28) that plays a pivotal role in regulating glucose and lipid metabolism, enhancing insulin sensitivity.
• It stimulates AMPK phosphorylation in liver and skeletal muscle, promoting glucose utilization and fatty-acid oxidation.
• Adiponectin antagonizes TNF-alpha by suppressing its expression and counteracting its pro-inflammatory effects in various tissues.
• Through a cAMP-dependent pathway, it inhibits endothelial NF-kappa-B signaling, contributing to its anti-atherogenic properties.
• This hormone may regulate cell growth, angiogenesis, and tissue remodeling by binding and sequestering growth factors with distinct complex-dependent affinities (LMW, MMW, HMW).
• Exclusively synthesized and secreted by adipocytes, adiponectin circulates in plasma and exerts systemic metabolic effects.
• Keywords include Collagen, Hormone, Secreted, Glycoprotein, and it is associated with diabetes mellitus and obesity.

Experimental Guidance and Technical Tips

• The immunogen used covers amino acids 19-244, which spans most of the mature adiponectin sequence, potentially ensuring detection of multiple complex forms.
• For WB, a band at approximately 26 kDa is expected under reducing conditions; consider testing various tissue lysates (e.g., adipose tissue, plasma) and validating in human, mouse, or rat samples given the cross-reactivity.
• In IHC-P, adiponectin expression is expected in adipocytes; use appropriate positive controls (e.g., adipose tissue sections) and consider antigen retrieval protocols.
• ELISA applications may benefit from pairing with a matched detection antibody; verify optimal dilutions empirically.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio provides validated antibody pairs, bulk polyclonal and monoclonal antibodies, and ancillary reagents for diagnostic development. This anti-adiponectin polyclonal antibody supports research in metabolic disorders, diabetes, and obesity, and CamelBio can assist in sourcing complementary raw materials or custom antibody services to streamline your assay development pipeline.