Anti-ADHFE1 Polyclonal Antibody for WB, ELISA - Q8IWW8

Core Product Specifications and Parameters

Biological Background: ADHFE1 Function and Localization

• ADHFE1 (alcohol dehydrogenase iron-containing protein 1), also known as HOT, ADH8, or HMFT2263, is a mitochondrial hydroxyacid-oxoacid transhydrogenase.
• Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA), coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG).
• Shows lower activity with substrates D,L-3-hydroxyisobutyrate and L-3-hydroxybutyrate (L-3-OHB), with approximately 10-fold reduced efficiency compared to GHB.
• Localized to the mitochondrion, consistent with its role in central metabolism.
• Expression is restricted to adult liver, indicating a tissue-specific metabolic function.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics studies.
• Belongs to the iron-containing alcohol dehydrogenase family, featuring alternative splicing.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant fusion protein covering amino acids 41–255 of human ADHFE1, encompassing a substantial portion of the mature protein; it is predicted to recognize the full-length endogenous protein.
• Validated for Western blot (WB) and ELISA; for WB, consider a starting dilution in the appropriate range for rabbit polyclonal antibodies and validate in human liver tissue or cell lines.
• Cross-reacts with mouse, allowing potential use in mouse liver samples; however, confirm specificity in your experimental system.
• Because ADHFE1 is mitochondrial, recommend using mitochondrial-enriched lysates for enhanced detection in WB.
• For ELISA applications, consider pairing with a matched detection antibody or optimizing coating antigen conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research labs, and institutes with streamlined access to a comprehensive portfolio of IVD raw materials. For metabolic enzyme targets like ADHFE1, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and support for rare target sourcing—ensuring you can secure every component needed from concept to clinic.