Anti-ADH7 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P40394

Core Product Specifications and Parameters

Biological Background: ADH7 Function and Localization

• ADH7, also known as alcohol dehydrogenase class 4 mu/sigma chain, gastric alcohol dehydrogenase, or retinol dehydrogenase, is a zinc-binding enzyme belonging to the alcohol dehydrogenase family.
• Catalyzes the NAD-dependent oxidation of all-trans-retinol, alcohol, and omega-hydroxy fatty acids, with a preference for all-trans-retinol, 2-hexenol, and long-chain omega-hydroxy fatty acids such as juniperic acid; also capable of reducing all-trans-retinal and aldehydes in vitro. Through these activities, it plays a critical role in retinoid metabolism, fatty acid omega-oxidation, and the elimination of cytotoxic aldehydes produced by lipid peroxidation.
  Related references: PMID:15369820 PMID:16787387 PMID:9600267
• Localizes predominantly to the cytoplasm.
• Preferentially expressed in the stomach.
• Contains a zinc-binding site and binds NAD, characteristic of medium-chain alcohol dehydrogenases.
• The protein has a molecular mass of ~41 kDa (41481 Da according to UniProt).
• Alternative splicing of the ADH7 gene produces multiple transcript variants.
• The three-dimensional structure of ADH7 has been experimentally determined.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human ADH7 protein (aa 1-386), so the polyclonal antibody may recognize multiple epitopes and could detect both native and denatured forms.
• For Western blotting, a band corresponding to ~41 kDa is expected; however, validate molecular weight markers and consider possible post-translational modifications that may shift migration.
• For immunofluorescence, anticipate cytoplasmic staining; validate with appropriate controls and consider using a nuclear counterstain.
• Cross-reactivity has been reported for mouse and rat; optimize species-specific detection conditions for each experimental model.
• Given the preferential stomach expression, stomach tissue lysates or sections are recommended as positive controls; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, providing this ADH7 antibody along with validated antibody pairs, bulk ancillary reagents, and custom development services. Whether you require optimized polyclonal antibodies for retinoid metabolism research or rare target reagent sourcing, our supply chain expertise supports your workflow from concept to clinic.