Anti-ADH5/GSNOR Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P11766

Core Product Specifications and Parameters

Biological Background: ADH5/GSNOR Function and Localization

• ADH5, also known as alcohol dehydrogenase class-3 or GSNOR, is a zinc-containing, NAD-dependent oxidoreductase that belongs to the alcohol dehydrogenase family.
• It catalyzes the oxidation of long-chain primary alcohols and the detoxification of formaldehyde via S-(hydroxymethyl)glutathione oxidation. Related references: PMID:8460164
• ADH5 is remarkably inefficient at oxidizing ethanol, distinguishing it from other class I alcohol dehydrogenases. Related references: PMID:8460164
• The enzyme also oxidizes long-chain omega-hydroxy fatty acids such as 20-HETE, generating both intermediate aldehydes and dicarboxylic acid products. Related references: PMID:16081420
• As GSNOR, it catalyzes the NADH-dependent reduction of S-nitrosoglutathione, thereby regulating protein S-nitrosylation and nitric oxide signaling.
• ADH5 is essential for cellular formaldehyde clearance, protecting against formaldehyde-induced DNA damage and cytotoxicity. Related references: PMID:33355142
• It is primarily localized in the cytoplasm and is associated with disease variants linked to dwarfism and intellectual disability.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human ADH5 protein (residues 1-374), suggesting the antibody may detect both native and denatured conformations.
• For Western blot (WB), a band around 40 kDa is expected; consider using total cell lysates from human, mouse, or rat tissues or cell lines as positive controls.
• In immunofluorescence/immunocytochemistry (IF/ICC), as ADH5 localizes to the cytoplasm, ensure proper fixation and permeabilization protocols to enable antibody access.
• For ELISA, the antibody can be used for detection or capture of ADH5 in solution; optimization of antibody concentration and antigen coating is recommended.
• Although cross-reactivity is listed for human, mouse, and rat, validation in the specific sample system of interest is advised to confirm reactivity and specificity.
• Consider performing a no-primary control and/or pre-adsorption with the immunogen to verify signal specificity in each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw material sourcing, offering comprehensive access to antibodies, antigens, and ancillary reagents for metabolic and detoxification enzyme research. In addition to this ADH5/GSNOR rabbit polyclonal antibody, we provide validated antibody pairs, optimized monoclonal antibodies, and bulk reagents tailored to your diagnostic development needs. Contact us to explore custom sourcing solutions for rare or high-demand molecular targets.