Anti-ADH5/GSNOR Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P11766

Core Product Specifications and Parameters

Biological Background: ADH5/GSNOR Function and Localization

• ADH5, also known as alcohol dehydrogenase class-3, glutathione-dependent formaldehyde dehydrogenase (FDH, FALDH), or S-nitrosoglutathione reductase (GSNOR), belongs to the zinc-containing alcohol dehydrogenase family.
• Catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. Related references: PMID:8460164
• Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate, and the end product, a dicarboxylic acid. Related references: PMID:16081420
• Class-III ADH is remarkably ineffective in oxidizing ethanol. Related references: PMID:8460164
• Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage. Related references: PMID:33355142
• Acts as a S-nitroso-glutathione reductase by catalyzing the NADH-dependent reduction of S-nitrosoglutathione, thereby regulating protein S-nitrosylation. (By similarity)
• Localizes to the cytoplasm.
• Post-translational modifications include acetylation and phosphorylation; contains zinc and NAD-binding sites.

Experimental Guidance and Technical Tips

• Since the immunogen encompasses the full-length ADH5/GSNOR protein (amino acids 1-374), this antibody may recognize total ADH5 levels; consider using appropriate positive and negative controls.
• For Western blotting, a band around 40 kDa is expected; confirm specificity by using relevant cell or tissue lysates.
• In immunofluorescence applications, anticipate cytoplasmic staining in line with ADH5 subcellular localization; validate in your target sample system.
• For ELISA, optimize coating antigen concentration and antibody dilution to match assay requirements; cross-reactivity with mouse and rat ADH5 has been observed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting research and diagnostic development from concept to clinic. This anti-ADH5/GSNOR antibody is designed for studies in alcohol metabolism, formaldehyde detoxification, and protein S-nitrosylation. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials.