Anti-ADH4 Rabbit Polyclonal Antibody for WB, ELISA - P08319

Core Product Specifications and Parameters

Biological Background: ADH4 Function and Localization

• ADH4 (alcohol dehydrogenase 4) is also known as alcohol dehydrogenase 2 (ADH2), alcohol dehydrogenase class II pi chain, and all-trans-retinol dehydrogenase, involved in the metabolism of retinol and fatty acids.
• Catalyzes NAD-dependent oxidation of all-trans-retinol and 9-cis-retinol, playing a critical role in retinoid metabolism.
  Related references: PMID:17279314
• Also oxidizes long-chain omega-hydroxy fatty acids such as 20-HETE, producing the intermediate aldehyde and dicarboxylic acid.
  Related references: PMID:16081420
• Can reduce benzoquinones, contributing to its broader oxidoreductase activity.
  Related references: PMID:10514444
• Localized to the cytoplasm, consistent with its metabolic functions.
• Contains metal-binding and zinc-binding motifs, indicative of its requirement for zinc as a cofactor.
• Subject to alternative splicing and phosphorylation, adding to its regulatory complexity.
• Proteomics identification and available 3D structures support its well-characterized nature.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human ADH4 protein (amino acids 1-380), suggesting the antibody can detect native ADH4 in WB under appropriate conditions.
• For WB, consider using reducing conditions and a suitable protein marker to confirm the ~40 kDa band.
• For ELISA, validate antibody specificity with recombinant ADH4 or cell lysates expressing ADH4.
• Given cross-reactivity with mouse, the antibody may be suitable for mouse tissue studies, but verify reactivity in your system.
• Always include positive and negative controls to ensure assay specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a comprehensive range of IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies for targets in metabolic pathways. This Anti-ADH4 Rabbit Polyclonal Antibody supports research in alcohol dehydrogenase and retinoid metabolism, and CamelBio can assist with bulk orders and ancillary reagents to streamline your assay development and production.