Anti-ADH1B Rabbit Polyclonal Antibody for WB, ELISA - P00325

Core Product Specifications and Parameters

Biological Background: ADH1B Function and Localization

• ADH1B, also known as alcohol dehydrogenase 1B or subunit beta, is an all-trans-retinol dehydrogenase belonging to the alcohol dehydrogenase family.
• Participates in retinoid metabolism by catalyzing the NAD-dependent oxidation of all-trans-retinol and its derivatives, and can also mediate the NADH-dependent reduction of all-trans-retinal derivatives. Related references: PMID:15369820, PMID:16787387
• Shows higher catalytic efficiency in the oxidative direction and displays similar affinity for all-trans-4-hydroxyretinol and all-trans-4-oxoretinal. Related references: PMID:15369820, PMID:16787387
• Subcellularly localized to the cytoplasm.
• A zinc-dependent and NAD-binding oxidoreductase.
• Subject to acetylation and phosphorylation, as detected in proteomic studies.
• Multiple isoforms can be generated through alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen comprises the C-terminal region (amino acids 206–375) of human ADH1B; epitopes within this fragment may be conserved across species, as evidenced by the reported cross-reactivity with rat ADH1B.
• For Western blot applications, a denaturing SDS-PAGE under reducing conditions is typically appropriate; the expected protein weight is approximately 40 kDa.
• The antibody has been validated for WB and ELISA; users should optimize dilutions and validate performance in their specific sample matrix and assay setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research institutes with comprehensive IVD raw material solutions. This anti-ADH1B polyclonal antibody serves as a tool for exploring retinoid metabolism, alcohol-linked liver pathologies, and related biomarker research. Alongside antibodies, we supply validated antibody pairs, bulk ancillary reagents, and can assist with sourcing rare-target raw materials—supporting your projects from initial concept through clinical application.