Anti-ADH1A/ADH1B/ADH1C Rabbit Polyclonal Antibody for WB - P07327 / P00325 / P00326

Core Product Specifications and Parameters

Biological Background: ADH1A/ADH1B/ADH1C Function and Localization

• ADH1A encodes the alpha subunit of alcohol dehydrogenase 1A, a cytoplasmic enzyme responsible for oxidizing alcohols (UniProt P07327).
• It catalyzes the oxidation of primary and secondary alcohols, with ethanol serving as a very poor substrate (PubMed:2738060). Related references: PMID:2738060
• ADH1A is a member of the class I alcohol dehydrogenase gene family, which includes ADH1B and ADH1C; this antibody recognizes all three isoforms.
• The protein localizes to the cytoplasm and functions in a zinc- and NAD-dependent manner, undergoing post-translational modifications such as acetylation and phosphorylation.
• Keywords from UniProt include metal-binding, NAD, oxidoreductase, zinc, and phosphoprotein, reflecting its catalytic and regulatory properties.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from a conserved region (amino acids 270–370) of human ADH1A/ADH1B/ADH1C, which may account for cross-reactivity with mouse and rat orthologs; consider validation in the relevant sample system.
• For Western blotting, use whole-cell lysates from human, mouse, or rat tissues; the predicted molecular weight of ADH1A is approximately 40 kDa, but multiple bands may appear due to isoform similarities.
• In immunofluorescence (IF-P), anticipate cytoplasmic staining; optimize fixation and permeabilization conditions to avoid false negatives.
• For ELISA, evaluate the antibody in direct or sandwich formats; pairing with a detection antibody specific to the ADH1 subfamily may improve specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for metabolic and liver function research. This ADH1A/ADH1B/ADH1C antibody supports studies into alcohol dehydrogenase biology, while our supply chain expertise ensures reliable access to rare targets from concept to clinic.