Anti-ADGRB3 Rabbit Polyclonal Antibody for WB, ELISA - O60242

Core Product Specifications and Parameters

Biological Background: ADGRB3 Function and Localization

• ADGRB3 (Adhesion G protein-coupled receptor B3) is a member of the adhesion GPCR family, characterized by a large N-terminal extracellular domain with thrombospondin repeats, a seven-transmembrane region, and a cytoplasmic tail.
• It plays a critical role in synapse formation and dendritic spine morphogenesis through ELMO1/RAC1-dependent pathways (By similarity).
• Additionally, ADGRB3 facilitates myoblast fusion, underscoring its involvement in actin cytoskeleton remodeling. Related references: PMID:24567399
• The protein is predominantly expressed on the cell membrane of neurons and cardiomyocytes, consistent with its function in the brain and heart.
• Post-translational modifications, including glycosylation and phosphorylation, along with alternative splicing, generate functionally distinct isoforms and regulate receptor activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 70-280 in the extracellular domain, a region expected to be well surface-exposed; this antibody may recognize both native and denatured forms.
• For Western blot, consider loading 20–50 µg of total protein from human brain or heart lysates and using a gradient gel (e.g., 4–12%) to resolve the 172 kDa band; validate antibody specificity with a blocking peptide or knockdown controls.
• In ELISA, the antibody can be used as a capture or detection reagent; pair with a recombinant ADGRB3 standard and optimize coating concentrations to establish a standard curve.
• Due to the large protein size, ensure proper transfer conditions (e.g., extended transfer time or wet transfer) to avoid poor membrane retention.

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