Anti-ADGRA3 Rabbit Polyclonal Antibody for WB, ELISA - Q8IWK6

Core Product Specifications and Parameters

Biological Background: ADGRA3 Function and Localization

• ADGRA3 (adhesion G protein-coupled receptor A3) is also known as GPR125, PGR21, and TEM5L, and belongs to the adhesion GPCR family characterized by long N-terminal extracellular domains with adhesion motifs.
• It is an orphan receptor, meaning its endogenous ligand is currently unknown, and it may play a role in the planar cell polarity pathway.
• The protein contains multiple leucine-rich repeats and an immunoglobulin domain, which are common structural features of adhesion GPCRs and are thought to mediate cell-cell interactions.
• ADGRA3 is predicted to be a transmembrane protein with seven transmembrane helices, a signal peptide, and is localized at the cell membrane.
• According to UniProt keywords, ADGRA3 is subject to alternative splicing, is glycosylated, and contains disulfide bonds, which may affect its stability and function.
• It serves as a potential research target for studying GPCR signaling, planar cell polarity, and related developmental pathways.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to amino acids 370-600 of human ADGRA3, a region likely within the extracellular domain; for Western blot, consider using positive controls from human cell lines known to express ADGRA3.
• As a polyclonal antibody, it may detect multiple bands due to alternative splicing or post-translational modifications; validate specificity in the relevant sample system.
• For ELISA applications, the recombinant protein immunogen can be used as a standard; optimize coating and detection conditions in your lab.
• Cross-reactivity with mouse and rat has been confirmed by the supplier, enabling its use in rodent models; still, verify performance in your specific experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing a comprehensive range of IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies like this ADGRA3 antibody. With expertise in rare target raw-material sourcing, we can also supply bulk ancillary reagents and tailored procurement solutions to accelerate your projects in GPCR and cellular polarity research. Contact us to streamline your supply chain with reliable, research-grade materials.