Anti-Adenosylhomocysteinase (AHCY/SAHH) Rabbit Monoclonal Antibody for WB and ELISA - P23526

Core Product Specifications and Parameters

Biological Background: AHCY Function and Localization

• Adenosylhomocysteinase (AHCY), also referred to as SAHH or adoHcyase, is a key enzyme in one-carbon metabolism that catalyzes the reversible hydrolysis of S-adenosyl-L-homocysteine (SAH) to adenosine and homocysteine. Related references: PMID:10933798
• AHCY plays a critical role in regulating the intracellular concentration of SAH, a potent inhibitor of methyltransferases, thereby influencing methylation reactions across the cell.
• The enzyme is ubiquitously expressed and localizes to multiple subcellular compartments, including the cytoplasm, nucleus, endoplasmic reticulum, and melanosomes, reflecting its involvement in diverse cellular processes.
• AHCY contains an NAD+ binding domain and functions as a hydrolase, requiring NAD+ as a cofactor for its enzymatic activity.
• Post-translational modifications of AHCY, including acetylation, phosphorylation, and hydroxylation, may regulate its activity or subcellular localization.
• Mutations in AHCY are associated with disease variants, linking the protein to disorders of methionine metabolism and homocysteine accumulation.
• Alternative splicing of the AHCY gene generates multiple transcript variants, potentially producing isoforms with distinct functions or localizations.
• The protein also binds copper ions, which might have implications for its function or stability.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 300-400) of human AHCY. For Western blot (WB), a band around 48 kDa is expected; however, validate the antibody’s specificity using appropriate positive and negative controls (e.g., knockdown samples or tissues with known expression levels).
• Although this rabbit monoclonal antibody is suitable for WB and ELISA, consider optimizing antibody dilution, incubation conditions, and detection methods for your specific experimental system.
• Cross-reactivity with mouse and rat AHCY suggests conserved epitope sequences; nevertheless, confirm reactivity in your target species’ samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner, offering diagnostic manufacturers and researchers access to validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our AHCY antibody supports studies in one-carbon metabolism and related disease research, helping you bridge the gap from concept to clinic with reliable raw materials.