Anti-Adenosine Deaminase (ADA) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P00813

Core Product Specifications and Parameters

Biological Background: Adenosine Deaminase Function and Localization

• Catalyzes hydrolytic deamination of adenosine and 2-deoxyadenosine, essential for purine metabolism and adenosine homeostasis.
  Related references: PMID:16670267, PMID:23193172, PMID:26166670
• Binds DPP4 to positively regulate T-cell coactivation and lymphocyte-epithelial cell adhesion.
  Related references: PMID:20959412, PMID:11772392
• Enhances dendritic cell immunogenicity by modulating costimulatory molecules and cytokines/chemokines (By similarity).
• Promotes CD4+ T-cell differentiation and proliferation.
  Related references: PMID:20959412
• Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A by increasing ligand affinity.
  Related references: PMID:23193172
• Stimulates plasminogen activation.
  Related references: PMID:15016824
• Plays a role in male fertility and early postimplantation embryonic development.
  Related references: PMID:21919946, PMID:26166670
• Localizes to cell membrane, cell junctions, cytoplasmic vesicles, cytoplasm, and lysosomes; widely expressed, with high levels in T-lymphocytes and gastrointestinal tissues at weaning.
  Related references: PMID:20959412

Experimental Guidance and Technical Tips

• The immunogen consisting of full-length (1–363) recombinant ADA may provide broad epitope coverage; consider optimization for each application.
• For WB, the predicted molecular weight is approximately 41 kDa; use appropriate gel percentage and validate with positive control lysates.
• In IF/ICC, ADA localizes to multiple compartments; include appropriate controls and consider permeabilization conditions.
• For ELISA, validate with recombinant protein or known positive samples; titration is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for immunodiagnostic development. This ADA polyclonal antibody is ideal for research in purine metabolism, immune regulation, and related disorders. We also support rare target raw-material sourcing to accelerate your assay development.