Anti-Adenosine Deaminase (ADA) Rabbit Monoclonal Antibody for WB, ELISA - P00813

Core Product Specifications and Parameters

Biological Background: Adenosine Deaminase Function and Localization

• Adenosine deaminase (ADA), also termed adenosine aminohydrolase, catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine, serving a central role in purine metabolism and adenosine homeostasis (PubMed:16670267, 23193172, 26166670). Related references: PMID:16670267 PMID:23193172 PMID:26166670
• ADA positively regulates T-cell coactivation by binding DPP4/CD26, thereby modulating lymphocyte-epithelial cell adhesion and enhancing CD4+ T-cell differentiation and proliferation (PubMed:20959412, 11772392). Related references: PMID:20959412 PMID:11772392
• It enhances dendritic cell immunogenicity by influencing costimulatory molecule expression and cytokine secretion (By similarity).
• The enzyme acts as a positive allosteric modulator of adenosine receptors ADORA1 and ADORA2A, increasing their ligand affinity (PubMed:23193172). Related references: PMID:23193172
• ADA stimulates plasminogen activation and contributes to male fertility and early postimplantation embryonic development (PubMed:15016824, 21919946, 26166670). Related references: PMID:15016824 PMID:21919946 PMID:26166670
• Additionally, ADA deaminates cordycepin (3'-deoxyadenosine), a fungal metabolite with antitumor, antibacterial, and antiviral properties (PubMed:26038697). Related references: PMID:26038697
• ADA is a zinc-binding enzyme found at the cell membrane, cell junctions, cytoplasm, lysosomes, and cytoplasmic vesicle lumens; it is ubiquitously expressed with particularly high levels in T-lymphocytes (PubMed:20959412), and its deficiency causes severe combined immunodeficiency (SCID). Related references: PMID:20959412

Experimental Guidance and Technical Tips

• The predicted molecular weight is ~41 kDa; for Western blot, consider using appropriate positive controls and optimizing protein loading.
• The immunogen sequence (aa 250–350) is conserved among human, mouse, and rat, supporting the observed cross-reactivity.
• For ELISA, validate the antibody in your specific sample matrix and coating conditions, as performance may vary.
• Tissue lysates from lymphoid organs (e.g., spleen, thymus) are likely to yield stronger signals due to high ADA expression in T-lymphocytes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw-material procurement by offering validated antibody pairs, monoclonal antibodies, and bulk ancillary reagents. For researchers investigating adenosine metabolism, T-cell activation, or SCID, this anti-ADA monoclonal antibody is available alongside complementary products and custom sourcing support. Let us bridge your concept-to-clinic journey with reliable supply chains and technical expertise.