Anti-ADCYAP1R1 (PAC1) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P41586

Core Product Specifications and Parameters

Biological Background: ADCYAP1R1 Function and Localization

• ADCYAP1R1 (PAC1) is a class B G protein-coupled receptor (GPCR) that functions as the high-affinity receptor for the pituitary adenylate cyclase-activating polypeptide (PACAP), binding both PACAP27 and PACAP38 isoforms (PubMed:32004469, PubMed:32047270, PubMed:35477937).
  Related references: PMID:32004469 PMID:32047270 PMID:35477937
• Ligand engagement triggers G protein-mediated activation of adenylyl cyclase and the cAMP-dependent signaling cascade, modulating diverse downstream effectors (PubMed:32004469, PubMed:32047270, PubMed:33715378).
  Related references: PMID:32004469 PMID:32047270 PMID:33715378
• The receptor is involved in neuroendocrine regulation, with proposed roles in controlling the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine.
• In the gastrointestinal system, ADCYAP1R1 mediates smooth muscle relaxation and secretory processes (PubMed:32047270, PubMed:33715378).
  Related references: PMID:32047270 PMID:33715378
• The protein is localized to the cell membrane, featuring seven transmembrane domains typical of GPCRs.
• Expression is predominantly in the brain, with lower levels detectable in lung, liver, thymus, spleen, pancreas, and placenta.
• ADCYAP1R1 is associated with developmental processes, cell differentiation, and spermatogenesis, and is subject to alternative splicing and post-translational modifications such as disulfide bond formation, glycosylation, and phosphorylation.
• Structural studies have resolved its 3D architecture, revealing key features for ligand recognition and signal transduction.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (amino acids 25–140), which is exposed on the cell surface; this design favors detection of the native receptor by WB and IF/ICC.
• For Western blotting, anticipate a band near 53 kDa in lysates from human, mouse, or rat tissues; validate expression in brain samples where the target is abundant.
• In immunofluorescence/immunocytochemistry, optimize permeabilization and antibody concentration to visualize membrane-localized PAC1 in neuronal cell lines or primary cultures.
• As a polyclonal antibody, it may generate additional bands; include appropriate controls and consider using knockout or knockdown validation to confirm specificity.
• For ELISA applications, the antibody can be used as a capture or detection reagent; pair with a matched monoclonal antibody for sandwich immunoassays if quantitative detection is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by providing one-stop access to validated antibodies, antigen-grade proteins, and ancillary reagents for diagnostic development. This ADCYAP1R1 antibody supports neuroscience and GPCR research, and we can supply complementary products such as optimized monoclonal/polyclonal pairs, bulk recombinant proteins, and rare target sourcing tailored to your workflow. From concept to clinic, CamelBio bridges your supply needs with technical expertise.