Anti-ADCY2 Rabbit Polyclonal Antibody for WB, ELISA - Q08462

Core Product Specifications and Parameters

Biological Background: ADCY2 Function and Localization

• ADCY2 encodes adenylate cyclase type 2, a member of the adenylyl cyclase family, which catalyzes the conversion of ATP to cyclic AMP (cAMP) upon activation by G protein-coupled receptors (PubMed:15385642). Related references: PMID:15385642
• The enzyme functions in signaling cascades downstream of muscarinic acetylcholine receptors and contributes to the regulation of gene expression, including increased IL6 production (By similarity).
• Alternative splicing generates multiple transcript variants, indicating potential isoform diversity (based on UniProt keyword).
• Subcellularly, ADCY2 localizes to the cell membrane and cytoplasm, consistent with a transmembrane protein that cycles between compartments.
• Post-translational modifications include phosphorylation (phosphoprotein) and N-linked glycosylation (glycoprotein), which may regulate activity and stability.
• Tissue expression is highest in the brain, particularly the caudate nucleus, cerebellum, and hippocampus; at the protein level, it is also detected in the zona glomerulosa and zona fasciculata of the adrenal gland (PubMed:11549699). Related references: PMID:11549699

Experimental Guidance and Technical Tips

• For Western blotting, a band around 124 kDa is expected. Use whole cell lysates from brain or adrenal tissues (human, mouse, rat) known to express ADCY2.
• For ELISA, validate the antibody’s specificity using recombinant ADCY2 protein or cell lysates from appropriate species.
• Consider optimizing antibody dilution and incubation conditions for each application, as performance can vary with sample type and assay format.
• Since ADCY2 is a transmembrane protein, use lysis buffers containing detergents (e.g., 1% NP-40 or Triton X-100) to ensure efficient solubilization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, from concept to clinic. For ADCY2 research, we offer validated polyclonal antibodies and can source rare targets and ancillary reagents to accelerate your diagnostic development. Our supply capabilities include optimized antibody pairs and bulk reagents tailored to your needs.