Anti-ADCY10/SAC Rabbit Polyclonal Antibody for WB and ELISA - Q96PN6

Core Product Specifications and Parameters

Biological Background: ADCY10 Function and Localization

• ADCY10 (adenylate cyclase type 10) is a soluble adenylyl cyclase (sAC) also designated SAC, HCA2, or testicular soluble adenylyl cyclase. Alternative splicing generates multiple protein isoforms.
• It acts as a bicarbonate and CO₂ sensor, catalyzing the formation of the second messenger cAMP from ATP (PubMed:12609998, 15659711, 24567411).
  Related references: PMID:12609998, PMID:15659711, PMID:24567411
• ADCY10 plays an essential role in spermatogenesis: it generates cAMP that activates nuclear factors required for sperm maturation in the epididymis and triggers capacitation prior to fertilization.
• It is also involved in the regulation of ciliary beat frequency in airway epithelial cells (PubMed:17591988).
  Related references: PMID:17591988
• Protein expression is highest in testis and airway epithelial cells; it is detectable at lower levels in multiple tissues including brain, heart, kidney, liver, lung, and pancreas.
• Subcellularly, ADCY10 localizes to the cell membrane, cytoplasm, cytoskeleton, perinuclear region, nucleus, cilium, and mitochondria.
• The enzyme requires divalent cations (Mg²⁺ or Mn²⁺) for activity and possesses ATP‑binding sites; several alternatively spliced variants exist.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal region (amino acids 1–290) of human ADCY10; the antibody may recognize full‑length and some truncated isoforms.
• For Western blotting, the predicted molecular weight is 187 kDa. Use a 6–8% SDS‑PAGE gel and appropriate high‑molecular‑weight markers to achieve optimal separation. Consider extended transfer times for such a large protein.
• For ELISA, preliminary checkerboard titrations against recombinant or native ADCY10 are advised to determine optimal coating and antibody concentrations.
• The antibody has documented reactivity with human and mouse samples; validate performance in your experimental system, particularly if working with weakly expressing tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw‑material procurement by supplying validated antibody pairs, optimized recombinant proteins, and ancillary reagents for assay development. For targets like ADCY10—central to cAMP signaling and reproductive biology—our portfolio includes polyclonal and monoclonal antibodies, bulk antigen‑grade proteins, and rare‑target sourcing support. Partner with CamelBio to bridge discovery and clinical manufacturing with reliable, research‑grade materials.