Anti-ADAR1 Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P55265

Core Product Specifications and Parameters

Biological Background: ADAR1 Function and Localization

• ADAR1 (Double-stranded RNA-specific adenosine deaminase) catalyzes the hydrolytic deamination of adenosine to inosine in dsRNA (A-to-I editing), affecting mRNA translation, splicing, stability, and microRNA function. Related references: PMID:12618436, PMID:7565688, PMID:7972084
• It edits both viral and cellular RNAs, including neurotransmitter receptors (GRIA2, HTR2C, GABRA3) and viral genomes (HCV, HDV, HIV-1), exhibiting either proviral or antiviral effects depending on the context.
• ADAR1 is expressed as multiple isoforms; isoform 1 is interferon-inducible and contains two Z-DNA-binding domains, while isoform 5 is constitutively expressed and lacks the N-terminal Z-DNA-binding domains.
• Subcellular localization includes cytoplasm, nucleus, and nucleolus, reflecting its diverse roles in RNA processing and antiviral innate immunity.
• Key post-translational modifications include phosphorylation, methylation, and sumoylation (as indicated by keywords), regulating its activity and interactions.
• Tissue specificity: ubiquitously expressed with highest levels in brain and lung; isoform 5 is upregulated in astrocytomas and its expression increases with tumor severity. Related references: PMID:7972084
• Functions in innate immunity by suppressing PKR activation and through editing-dependent mechanisms, highlighting its role in antiviral defense and autoimmune disorders (Aicardi-Goutières syndrome).

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (aa 200-299) of human ADAR1 isoform 1 (NP_001102.2). Check sequence homology with mouse and rat ADAR1 to confirm expected cross-reactivity.
• For Western blot, ADAR1 has a predicted molecular weight of ~136 kDa; ensure appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• For immunofluorescence and immunohistochemistry, consider that ADAR1 localizes to both cytoplasm and nucleus; validate subcellular staining patterns in relevant cell lines or tissues.
• ELISA can be used for quantification, but optimal coating antigen and detection conditions should be empirically determined.
• Because ADAR1 is interferon-inducible, stimulation with interferon may enhance expression levels in certain cell types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibodies like this anti-ADAR1 polyclonal antibody. We support your projects in RNA biology and infectious disease with bulk reagents, custom antibody development, and technical consulting from concept to clinic.