Anti-ADAR1 Rabbit Monoclonal Antibody for WB, ELISA - P55265

Core Product Specifications and Parameters

Biological Background: ADAR1 Function and Localization

• ADAR1 (Double-stranded RNA-specific adenosine deaminase, p136, IFI-4) catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (A-to-I RNA editing), which can alter coding sequences, splicing, RNA stability, and microRNA function (PubMed:12618436, PubMed:7565688, PubMed:7972084). Related references: PMID:12618436 PMID:7565688 PMID:7972084
• Its cellular substrates include GRIA2, HTR2C, GABRA3, and BLCAP, where site-specific editing changes amino acids and modulates receptor activity.
• It edits viral RNAs from HCV, VSV, MV, HDV, and HIV-1, exhibiting either proviral or antiviral effects through editing-dependent and editing-independent mechanisms (e.g., suppression of PKR activation).
• Localizes to the cytoplasm, nucleus, and nucleolus, consistent with its roles in RNA processing and innate immunity.
• Ubiquitously expressed with highest levels in brain and lung; isoform 5 is upregulated in astrocytomas and correlates with tumor aggressiveness.
• Contains Z-DNA binding domains, a deaminase domain, and dsRNA binding motifs; classified under hydrolases, RNA-binding proteins, and is implicated in Aicardi-Goutières syndrome and antiviral defense.

Experimental Guidance and Technical Tips

• The immunogen spans residues 200-300 of human ADAR1; confirm reactivity in your specific sample type and application.
• For Western blot, the predicted molecular weight is ~136 kDa; use appropriate gel acrylamide percentage and long transfer times to ensure proper detection of high-molecular-weight proteins.
• ELISA applications may require optimization of coating antigen, antibody dilution, and detection conditions to achieve optimal signal-to-noise ratio.
• ADAR1 expression can be induced by interferon; consider stimulating cells with interferon if basal levels are low.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw material sourcing, offering high-quality monoclonal antibodies, antigen pairs, and ancillary reagents for diagnostic development. This anti-ADAR1 rabbit monoclonal antibody supports research in RNA editing, antiviral immunity, and cancer biology. Whether you need validated antibody pairs, bulk raw materials, or procurement of rare targets, CamelBio bridges the gap from concept to clinic.