Anti-ADAMTSL1 Rabbit Polyclonal Antibody for WB and ELISA - Q8N6G6

Core Product Specifications and Parameters

Biological Background: ADAMTSL1 Function and Localization

• ADAMTSL1 (ADAMTS-like protein 1), also known as Punctin-1, is a secreted glycoprotein belonging to the ADAMTS family.
• It is localized to the extracellular space and extracellular matrix (ECM).
• Primarily expressed in adult skeletal muscle, suggesting a role in muscle ECM organization and integrity.
• The protein contains immunoglobulin (Ig) domains and thrombospondin type-1 repeats, structural features typical of ECM-associated proteins.
• Alternative splicing generates multiple isoforms of ADAMTSL1.
• The target protein appears at approximately 62 kDa in Western blotting under standard conditions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the conserved C-terminal region (residues 1483–1762), which likely accounts for the cross-reactivity observed with mouse and rat samples.
• For Western blotting, use reducing conditions and include appropriate positive controls such as skeletal muscle lysate. The expected band is around 62 kDa.
• For ELISA applications, the polyclonal nature may enhance sensitivity. Consider pairing with a complementary capture antibody for sandwich ELISA development.
• As a secreted matrix protein, ADAMTSL1 may be enriched in tissue extracts or conditioned media rather than cytosolic fractions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, including validated antibodies for ECM and muscle biology targets like ADAMTSL1. Our one-stop model encompasses bulk antibodies, custom polyclonal development, and ancillary reagents, streamlining development from concept to clinic. Reach out to explore tailored sourcing solutions for rare targets.