Anti-ADAMTS5 Rabbit Monoclonal Antibody for WB and ELISA - Q9UNA0

Core Product Specifications and Parameters

Biological Background: ADAMTS5 Function and Localization

• ADAMTS5, also known as aggrecanase-2, is a secreted metalloproteinase belonging to the ADAMTS family, with alternative names including ADMP-2 and ADAMTS11.
• It functions as an extracellular matrix-degrading enzyme with proteolytic activity toward hyalectan chondroitin sulfate proteoglycans such as aggrecan (ACAN), versican (VCAN), brevican (BCAN), and neurocan (NCAN), cleaving at Glu-Xaa motifs. Related references: PMID:16133547, PMID:18992360
• ADAMTS5 plays a critical role in embryonic development, including limb and cardiac morphogenesis, and facilitates skeletal muscle development by remodeling versican in the pericellular matrix of myoblasts.
• It is involved in T-lymphocyte migration from draining lymph nodes following viral infection and contributes to brown adipose tissue development and browning of white adipose tissue.
• The protein localizes to the extracellular space and extracellular matrix, secreted after proteolytic removal of its prodomain.
• Tissue expression is low; detected primarily in placenta, but also in heart, brain, cervix, uterus, and cartilage, with elevated levels in arthritic joint capsule.
• Structural features include a signal peptide, prodomain, catalytic metalloproteinase domain with zinc-binding motif, disintegrin-like domain, thrombospondin type-1 repeats, and a cysteine-rich domain; it is a glycoprotein processed by cleavage on basic residues.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 601-700 of human ADAMTS5 (NP_008969.2); consider epitope accessibility in proximity to the C-terminal thrombospondin repeats when designing detection strategies.
• For Western blotting, ADAMTS5 has a predicted molecular weight of approximately 102 kDa; use appropriate gel acrylamide percentage (e.g., 8–10%) and ensure sufficient transfer time for high-molecular-weight proteins.
• This antibody is validated for ELISA; if developing a sandwich immunoassay, consider screening for a matched pair with a capture antibody recognizing a distinct, non-overlapping epitope.
• Reported cross-reactivity with mouse ADAMTS5 allows for translational studies; always validate specificity in the relevant species-derived sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research institutes, and laboratories a streamlined, end-to-end raw-material supply chain—from concept to clinic. As a dedicated IVD partner, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets like ADAMTS5. Connect with us to advance your ECM and protease research with reliable, high-quality immunoreagents.