Anti-ADAMTS5 Polyclonal Antibody for WB, ELISA - Q9UNA0

Core Product Specifications and Parameters

Biological Background: ADAMTS5 Function and Localization

• ADAMTS5, also known as aggrecanase-2, ADAM-TS 11, or ADMP-2, is a secreted metalloproteinase belonging to the ADAMTS family of extracellular proteases.
• ADAMTS5 functions as an extracellular matrix (ECM) degrading enzyme that shows proteolytic activity toward hyalectan chondroitin sulfate proteoglycans (CSPGs) including ACAN, VCAN, BCAN, and NCAN, playing a key role in cartilage turnover and connective tissue remodeling. Related references: PMID:16133547 PMID:18992360
• The protein localizes to the extracellular space and extracellular matrix, where it remodels pericellular environments during development and tissue maintenance.
• ADAMTS5 contains metalloprotease, disintegrin, and thrombospondin type‑1 repeat domains; catalytic activity requires zinc binding, and the enzyme is initially produced as a zymogen.
• Tissue expression is detected at low levels in placenta, heart, brain, cervix, uterus, bladder, esophagus, and cartilage, with increased abundance in arthritic joint capsules.
• Through versican cleavage, ADAMTS5 contributes to embryonic limb and cardiac morphogenesis and facilitates myoblast fusion during skeletal muscle development and regeneration.
• ADAMTS5 also participates in T‑lymphocyte migration from draining lymph nodes following viral infection.
• The protein is subject to cleavage on pairs of basic residues and contains multiple disulfide bonds and glycosylation sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 601–700 of human ADAMTS5 (NP_008969.2). When designing detection strategies, verify that the targeted sequence is conserved in your species of interest, as cross‑reactivity is reported for human, mouse, and rat.
• For Western blotting, the predicted molecular weight is approximately 101 kDa. Optimize gel percentage (e.g., 8–10% polyacrylamide) and transfer conditions to ensure efficient blotting of this relatively large protein. Include a positive control lysate from placenta or cartilage tissue.
• Because this is a polyclonal antibody, it may recognize multiple epitopes; confirm specificity by parallel detection with an independent method (e.g., siRNA knockdown or antigen competition) if needed.
• For ELISA, pair with a suitable detection antibody and consider using recombinant ADAMTS5 or tissue extracts as capture antigen. Titrate the antibody to determine an optimal working concentration.
• Although the antibody is validated for WB and ELISA by the manufacturer, performance in other applications (e.g., IHC, IF) should be tested empirically under the relevant sample and buffer conditions.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a single access point for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents in bulk. For connective tissue and inflammation studies, we support sourcing of rare‑target reagents such as anti‑ADAMTS5 antibodies. Contact our team to streamline your raw‑material procurement and scale your diagnostic development projects.