Anti-ADAMTS4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O75173

Core Product Specifications and Parameters

Biological Background: ADAMTS4 Function and Localization

• ADAMTS4 (a disintegrin and metalloproteinase with thrombospondin motifs 4), also known as aggrecanase-1, is a zinc-dependent metalloprotease that cleaves aggrecan at the Glu373-Ala374 bond (also designated Glu392-Ala393). This activity is critical for cartilage degradation and is implicated in arthritic diseases. Related references: PMID:10356395, PMID:10827174
• Additionally, ADAMTS4 cleaves cartilage oligomeric matrix protein (COMP), further contributing to extracellular matrix remodeling. Related references: PMID:39672391
• The enzyme is secreted into the extracellular matrix where it mediates proteolysis.
• ADAMTS4 is predominantly expressed in brain, lung, and heart, with low expression in placenta and skeletal muscle. An isoform (isoform 2) is detected in osteoarthritic synovium. Related references: PMID:23897278, PMID:16723216
• Synthesized as an inactive zymogen, ADAMTS4 is activated by proteolytic removal of the prodomain at a dibasic site, a process essential for its enzymatic function.
• The protein undergoes glycosylation and contains multiple disulfide bonds, contributing to its structural stability and secretion.
• ADAMTS4 may play a role in neurodegeneration, as it has been associated with the exacerbation of Alzheimer disease pathology.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 74-318 of human ADAMTS4. This region spans part of the prodomain and the beginning of the catalytic domain. Researchers should consider that this antibody may recognize both the proform and the mature enzyme after prodomain removal, depending on the experimental conditions.
• For Western blot (WB), a band at approximately 90 kDa (full-length zymogen) is expected, with possible detection of processed forms at lower molecular weights.
• This antibody is suitable for WB, IF/ICC, and ELISA. Cross-reactivity with mouse and rat ADAMTS4 has been observed, making it useful for studies in rodent models. However, we recommend validating the antibody in the specific sample system and assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio of antibodies, recombinant proteins, and ancillary reagents. We support diagnostic manufacturers and researchers with validated antibody pairs, bulk monoclonal/polyclonal antibodies, and rare target sourcing to accelerate assay development. For studies involving ADAMTS4 and cartilage degradation or neurodegenerative diseases, CamelBio provides reliable raw materials and technical consultation from concept to clinic.