Anti-ADAMTS2 Rabbit Polyclonal Antibody for WB, ELISA - O95450

Core Product Specifications and Parameters

Biological Background: ADAMTS2 Function and Localization

• ADAMTS2 (A disintegrin and metalloproteinase with thrombospondin motifs 2), also known as procollagen I N-proteinase (PC I-NP), is a secreted metalloprotease belonging to the ADAMTS family.
• It plays a critical role in collagen fibril assembly by cleaving the N-terminal propeptides of type I and type II procollagens, a step essential for proper collagen maturation and deposition in the extracellular matrix.
• ADAMTS2 does not act on type III collagen, indicating substrate specificity among fibrillar collagens.
• Additionally, ADAMTS2 cleaves lysyl oxidase (LOX) downstream of its propeptide site, generating a short LOX form with reduced collagen-binding activity, thereby modulating collagen cross-linking. Related references: PMID:31152061
• The protein is highly expressed in tissues rich in fibrillar collagens, such as skin, bone, tendon, and aorta, with lower levels in thymus and brain.
• Subcellular localization is primarily extracellular, being secreted into the extracellular matrix where it performs its catalytic functions.
• ADAMTS2 is associated with collagen degradation and is implicated in Ehlers-Danlos syndrome (dermatosparactic type), a connective tissue disorder characterized by extreme skin fragility, underscoring its importance in matrix integrity.

Experimental Guidance and Technical Tips

• This polyclonal antibody is generated against a recombinant fusion protein corresponding to amino acids 1029-1213 of human ADAMTS2. For Western blot, consider the predicted molecular weight of approximately 135 kDa; however, post-translational modifications or alternative splicing may result in additional bands.
• The antibody shows cross-reactivity with human and rat ADAMTS2. Validate cross-reactivity in your specific tissue or cell lysate system, as expression levels may vary across different tissues.
• For ELISA applications, the immunogen region may serve as a suitable coating antigen to assess antibody titer and specificity. Consider optimizing coating concentration and blocking conditions empirically.
• Given the extracellular and secreted nature of ADAMTS2, consider analyzing conditioned media or tissue extracts for detection. Ensure appropriate sample preparation to avoid degradation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers and research institutions from concept to clinic. This anti-ADAMTS2 polyclonal antibody is offered as part of our broad portfolio of extracellular matrix and collagen research tools. With capabilities including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing, CamelBio streamlines your procurement process, helping you accelerate assay development and biomarker discovery.