Anti-ADAMTS13 Polyclonal Antibody for WB, IF/ICC, ELISA - Q76LX8

Core Product Specifications and Parameters

Biological Background: ADAMTS13 Function and Localization

• ADAMTS13 (A disintegrin and metalloproteinase with thrombospondin motifs 13), also known as von Willebrand factor-cleaving protease (vWF-CP), belongs to the ADAMTS family of metalloproteases.
• It specifically cleaves large von Willebrand factor (vWF) multimers in plasma into smaller, less thrombogenic forms, thereby regulating vWF-mediated platelet thrombus formation.
• This proteolytic activity is essential for maintaining the normal hemostatic balance and preventing excessive microvascular thrombosis.
• ADAMTS13 is primarily synthesized in the liver and secreted into the blood, where it circulates in plasma.
• The protein is a multidomain metalloprotease containing a signal peptide, propeptide, metalloprotease domain, disintegrin-like domain, thrombospondin type 1 repeats, and CUB domains; it requires zinc and calcium for activity.
• Post-translational modifications include glycosylation and disulfide bond formation, and it is known to exist in alternatively spliced isoforms.
• Keywords associated with ADAMTS13 include blood coagulation, hemostasis, hydrolase, metalloprotease, secreted, and disease variant, highlighting its role in thrombotic disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50–300 of human ADAMTS13, a region within the N-terminal part of the protein; consider that the polyclonal nature may lead to recognition of multiple epitopes, including potential isoforms or degradation products.
• For Western blotting, the predicted molecular weight is 154 kDa, but due to glycosylation and other modifications, the observed band may migrate at a higher or slightly different position; validate in the relevant sample system (e.g., human liver lysate or recombinant protein).
• In immunofluorescence/ICC, endogenous expression is expected in liver cells; use appropriate fixation/permeabilization methods and confirm signal specificity with controls.
• For ELISA, this antibody can be used as a capture or detection reagent, but optimal dilution and buffer conditions should be determined experimentally using purified antigen or known positive samples.
• Due to the antibody's cross-reactivity with mouse and rat, it may be suitable for preclinical models, but always verify species-specific reactivity and perform appropriate blocking steps.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, contract research organizations, and academic laboratories with a fully integrated procurement channel for IVD raw materials, including validated antibody pairs, bulk polyclonal and monoclonal antibodies, and ancillary reagents. For researchers studying thrombotic disorders and vWF-related pathways, our supply network can source anti-ADAMTS13 reagents like this product, along with complementary detection tools and technical consultation services. With end-to-end support from concept to clinic, CamelBio simplifies the sourcing of rare-target raw materials and accelerates assay development in hemostasis and coagulation research.