Anti-ADAM9 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q13443

Core Product Specifications and Parameters

Biological Background: ADAM9 Function and Localization

• ADAM9 (Disintegrin and metalloproteinase domain-containing protein 9), also known as Meltrin-gamma, MCMP, MDC9, is a member of the ADAM (a disintegrin and metalloprotease) family.
• It functions as a metalloprotease that cleaves and releases molecules involved in tumorigenesis and angiogenesis, including TEK, KDR, EPHB4, CD40, VCAM1, and CDH5.
• ADAM9 may mediate cell-cell and cell-matrix interactions and regulate cell motility through integrin binding.
• The protein may also act as an alpha-secretase for amyloid precursor protein (APP).
• Subcellular localization includes the cell membrane and secreted forms.
• Widely expressed in tissues, with isoform 2 highly expressed in liver and heart; also expressed in chondrocytes.
• Key post-translational modifications and structural features: it is a glycoprotein with disulfide bonds, and requires zinc for catalytic activity. It undergoes phosphorylation and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 29-210) of human ADAM9, which may target both pro- and mature forms. Consider performing validation experiments using relevant cell lysates or tissue samples to confirm specificity.
• For Western blot, the expected molecular weight is ~91 kDa (full-length), but processed forms may be detected due to shedding. Use appropriate controls and blocking conditions.
• The antibody is suitable for WB, IF-P, IHC-P, and ELISA; validate in your specific sample system, especially for species cross-reactivity (human, mouse, monkey).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents. For targets like ADAM9, which plays critical roles in cancer and inflammatory processes, we offer bulk sourcing options and custom procurement services to support your assay development and manufacturing needs.