Anti-ADAM8 Rabbit Polyclonal Antibody for WB and ELISA - P78325

Core Product Specifications and Parameters

Biological Background: ADAM8 Function and Localization

• ADAM8 (Disintegrin and metalloproteinase domain-containing protein 8), also designated CD156a or MS2, is a transmembrane glycoprotein belonging to the ADAM family of metalloproteases.
• The protein undergoes alternative splicing, generating multiple isoforms with potentially distinct functional properties.
• ADAM8 is characterized by its domain architecture, including a pro-domain, metalloprotease domain, disintegrin domain, and EGF-like repeat, with a zinc-binding catalytic site critical for its hydrolase activity.
• Functionally, ADAM8 may participate in the shedding of cell surface molecules and is implicated in leukocyte extravasation during inflammatory responses.
• Subcellular localization analysis places ADAM8 at the plasma membrane as a single-pass type I transmembrane protein, with its protease domain exposed extracellularly.
• Expression of ADAM8 is predominantly observed on immune cells, specifically neutrophils and monocytes, consistent with its role in inflammation.
• The protein is subject to post-translational modifications including glycosylation and disulfide bond formation, which may influence its stability and function.

Experimental Guidance and Technical Tips

• The immunogen used to generate this rabbit polyclonal antibody encompasses amino acids 17–300 of human ADAM8, covering the pro-domain and metalloprotease domain; this region may help target functionally relevant epitopes.
• For Western blot analysis, a band around 89 kDa is expected based on the protein weight; validate the detected band with appropriate positive and negative controls or siRNA knockdown if necessary.
• When performing ELISA, consider optimizing antibody coating concentration and pairing with a suitable detection antibody; cross-reactivity with mouse and rat ADAM8 allows for multi-species studies.
• This antibody is predicted to recognize native and denatured forms of ADAM8, but empirical testing under reducing vs. non-reducing conditions is recommended for your specific experimental setup.
• Given the metalloprotease nature of ADAM8, ensure that sample preparation buffers are compatible with preserving zinc-dependent activity if downstream functional assays are planned.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw-material procurement, connecting diagnostic manufacturers and research labs with reliable antibodies, antigens, and ancillary reagents. This ADAM8 rabbit polyclonal antibody exemplifies our commitment to providing well-characterized products for leukocyte biology and metalloprotease research. Beyond individual antibodies, we facilitate sourcing of validated antibody pairs, bulk-format polyclonal and monoclonal antibodies, and rare-target reagents—helping you streamline assay development from concept to clinic.