Anti-ADAM5 Rabbit Polyclonal Antibody for WB, ELISA - Q6NVV9

Core Product Specifications and Parameters

Biological Background: ADAM5 Function and Localization

• ADAM5, also known as tMDC II or ADAM5P, is a putative disintegrin and metalloproteinase domain-containing protein 5 belonging to the ADAM family.
• It is classified as a non-catalytic metalloprotease-like protein, lacking proteolytic activity due to the absence of essential catalytic residues.
• The protein features an EGF-like domain, which may mediate protein-protein interactions or contribute to cell adhesion processes.
• ADAM5 is highly expressed in the testis, suggesting a specialized role in male reproductive biology, possibly in spermatogenesis or fertilization.
• Alternative splicing generates multiple transcript variants of ADAM5, potentially expanding its functional diversity.
• As a reference proteome component, ADAM5 has been identified and annotated in high-throughput human proteome studies.
• The gene for ADAM5 is located on human chromosome 8, and its mouse ortholog shares significant sequence homology.

Experimental Guidance and Technical Tips

• For Western blot, a band around 47 kDa is expected based on the predicted molecular weight of ADAM5; use appropriate protein ladders for size confirmation.
• Since the immunogen is a synthetic peptide localized to residues 250–350, the antibody may recognize a specific epitope; consider peptide blocking controls to confirm specificity.
• The antibody is validated for human and mouse samples in WB and ELISA; for other species, cross-reactivity is not guaranteed and should be verified experimentally.
• As a polyclonal antibody, lot-to-lot consistency should be monitored; optimize the dilution factor for each new lot to maintain reproducibility.
• For ELISA, use the antibody as a capture or detection reagent, and validate with recombinant ADAM5 protein or cell lysates from testis tissue.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials and technical support from concept to clinic. For ADAM5 and related male reproductive targets, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, including rare target raw-material sourcing, to streamline your assay development. Let CamelBio be your trusted procurement partner, ensuring seamless access to high-quality reagents for your reproductive biology and diagnostic projects.