Anti-ADAM33 Polyclonal Antibody for WB, ELISA - Q9BZ11

Core Product Specifications and Parameters

Biological Background: ADAM33 Function and Localization

• ADAM33 (a disintegrin and metalloproteinase domain-containing protein 33) is a transmembrane metalloprotease that sheds the ectodomains of cell-surface proteins, modulating signaling pathways involved in angiogenesis, cell differentiation, inflammation, and proliferation.
• It acts as a constitutive sheddase of kit ligand/KITLG, releasing its soluble form to regulate downstream intracellular signaling. Related references: PMID:14676211
• In vascular smooth muscle cells, ADAM33 promotes and maintains a proliferative phenotype by activating the PI3K/AKT and ERK signaling cascades. Related references: PMID:33982767
• During airway vascular remodeling, ADAM33 enhances cell migration and regulates cytokine secretion through the same PI3K/AKT and ERK pathways. Related references: PMID:36091328
• It is primarily localized at the cell membrane.
• ADAM33 is widely expressed in human tissues except liver, with highest levels in placenta, lung, spleen, and veins.
• The protein contains several domains including a pro-domain cleaved during activation, a catalytic metalloprotease domain, a disintegrin domain, an EGF-like domain, and a transmembrane region. It is implicated in asthma susceptibility.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 500–700 of human ADAM33 (isoform NP_001269376.1). Researchers should verify sequence homology for cross-reactivity with mouse and rat, as stated.
• For western blotting, the predicted molecular weight is 88 kDa. Validate detection in appropriate cell or tissue lysates expressing ADAM33.
• ELISA can be considered for detecting native or recombinant ADAM33; optimal conditions should be determined experimentally.
• The antibody may recognize multiple isoforms due to alternative splicing; confirm specificity in the relevant model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For studies on ADAM33 in asthma, vascular remodeling, or angiogenesis, we provide reliable sourcing of this polyclonal antibody along with technical and consultative services. Contact us to streamline your assay development from concept to clinic.