Anti-ADAM23 Polyclonal Antibody for WB, ELISA - O75077

Core Product Specifications and Parameters

Biological Background: ADAM23 Function and Localization

• ADAM23, also known as MDC-3, is a disintegrin and metalloproteinase domain-containing protein 23 encoded by the ADAM23 gene in humans.
• It is a non-catalytic metalloprotease-like protein that may mediate cell-cell and cell-matrix interactions.
• ADAM23 belongs to the ADAM family, sharing structural features with other ADAM proteins but lacking catalytic activity.
• It localizes to the cell membrane and can be secreted, indicating both membrane-bound and soluble forms.
• The protein is highly expressed in the brain, particularly in the amygdala, caudate nucleus, hypothalamus, thalamus, cerebral cortex, and occipital pole, with weak expression in the heart.
• Post-translational modifications include alternative splicing, disulfide bonds, glycosylation, and cleavage on basic residues, reflecting its regulation and structural complexity.
• Keywords associated with ADAM23 include EGF-like domain, signal peptide, transmembrane helix, and cell adhesion, highlighting its role in neural and developmental processes.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 350-500, a region within the disintegrin-like domain, which may provide specificity for ADAM23 detection.
• For Western blot, consider using whole cell lysates from brain tissues or ADAM23-expressing cell lines; the predicted molecular weight is ~92 kDa.
• In ELISA applications, the antibody may be useful for detecting recombinant ADAM23 or endogenous protein in tissue homogenates, with optimization of coating and detection conditions.
• Given cross-reactivity with human, mouse, and rat, validate the antibody in the appropriate species-specific context, as sequence homology may influence binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop partner for IVD raw-material procurement, supporting your projects from antibody pairs to bulk ancillary reagents. For targets like ADAM23, implicated in neural cell adhesion, CamelBio offers optimized polyclonal and monoclonal antibody options, along with technical consultation and rare-target sourcing to streamline your diagnostic development workflow.