Anti-ADAM22 Rabbit Polyclonal Antibody for WB, ELISA - Q9P0K1

Core Product Specifications and Parameters

Biological Background: ADAM22 Function and Localization

• ADAM22 (Disintegrin and metalloproteinase domain-containing protein 22) is also known as MDC2, metalloproteinase-disintegrin ADAM22-3, and metalloproteinase-like, disintegrin-like, and cysteine-rich protein 2.
• It is a non-catalytic metalloprotease-like protein that functions as a probable ligand for integrins in the brain. Related references: PMID:19692335
• ADAM22 acts as a neuronal receptor for LGI1 and is involved in regulating cell adhesion, cell spreading, and inhibition of cell proliferation.
• Subcellularly, ADAM22 localizes to the cell membrane and is specifically found in cell projections such as axons.
• It is highly expressed in the brain and in some high-grade gliomas, with minimal expression in other tissues.
• The protein contains an EGF-like domain and disulfide bonds, and undergoes cleavage on a pair of basic residues; alternative splicing generates multiple isoforms.
• Mutations in ADAM22 are associated with epilepsy, highlighting its importance in neurological function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 223–400 within the extracellular domain; consider using this antibody to detect full-length ADAM22 and certain isoforms in WB.
• Validate the expected band around 100 kDa in relevant human brain or glioma cell lysates, and assess cross-reactivity in mouse and rat samples as indicated.
• For ELISA, the recombinant protein immunogen supports potential use; optimize coating and detection conditions with appropriate controls.
• Given ADAM22’s brain-specific expression, ensure sample preparation from neural tissues or cell lines to maximize signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-ADAM22 antibody supports neuroscience and glioma research, and our comprehensive supply chain ensures reliable access from concept to clinic. For customized antibody development or bulk orders, consult our technical services.