Anti-ADAM15 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q13444

Core Product Specifications and Parameters

Biological Background: ADAM15 Function and Localization

• ADAM15 (Disintegrin and metalloproteinase domain-containing protein 15) is a membrane-anchored metalloprotease belonging to the ADAM family, also referred to as MDC-15 or metargidin.
• It exhibits gelatinolytic and collagenolytic activity, contributing to extracellular matrix remodeling and wound healing.
• ADAM15 regulates cell adhesion through multiple mechanisms: it mediates T-cell interactions, inhibits beta-1 integrin-mediated adhesion and migration in airway smooth muscle, and suppresses cell motility on fibronectin by modulating alpha-v/beta-1 integrin expression via ERK1/2 inactivation.
• The protease cleaves cell adhesion molecules such as E-cadherin in response to growth factor deprivation, influencing epithelial integrity.
• Subcellularly, ADAM15 localizes to the endomembrane system, adherens junctions, cell projections (cilia, flagella), and secretory vesicles (acrosome), consistent with roles in adhesion, migration, and fertilization.
• The protein is ubiquitously expressed, with prominent detection in colon, small intestine, smooth muscle, glomerular mesangial cells, and vascular endothelium; overexpression in atherosclerotic lesions highlights a pathological role in vascular disease.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation; it is synthesized as a zymogen requiring proteolytic activation, and contains EGF-like and SH3-binding domains.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 540-700 of human ADAM15) encompasses the disintegrin and cysteine-rich domains, which are highly conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot, expect a band around 93 kDa; empirically optimize antibody dilution and incubation parameters for your specific samples.
• In immunofluorescence/immunocytochemistry, the antibody may label cell junctions, surface projections, or intracellular vesicles; appropriate fixation and permeabilization protocols should be validated.
• For ELISA, using the recombinant immunogen as a positive control can assist in assay development.
• As a polyclonal antibody, lot-to-lot consistency should be verified for each new batch to ensure reproducible performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-ADAM15 product. Our portfolio covers bulk ancillary reagents and custom sourcing for rare targets, facilitating seamless development from concept to clinic. Contact us to explore ADAM15-related reagents for your wound healing, cell adhesion, or angiogenesis projects.